Gene expression profiling as a means to assess radiation response patterns

We are attempting to determine whether there specific gene expression patterns that predict the response to radiation therapy, for diseases where local control issues persist. We have developed in-house protocols to perform microarray studies on RNA extracted from small amounts of formalin-fixed, paraffin-embedded (FFPE) tissues (photo on the left). We also use the NanoString platform for gene expression profiling with RNA extracted from tiny FFPE biopsy specimens (photo on the right). We are fortunate to have access to many clinical samples from both Memorial Sloan-Kettering Cancer Center, where Bindra did his residency training, and also from Yale.

Affymetix HTA microarray of RNA samples extracted from formalin-fixed paraffin-embedded (FFPE) tissue

Nanostring analysis of FFPE RNA specimens